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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sudeste. |
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Data corrente: |
09/09/2021 |
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Data da última atualização: |
10/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARRAIS, A. M.; MELLO, M. R. B. de; VERGANI, G. B.; FIGUEIRA, L. M.; ESTEVES, S. N.; PEREIRA, V. S. do A.; BARTLEWSKI, P. M.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. |
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Afiliação: |
ALINE MATOS ARRAIS, Universidade Federal Rural do Rio de Janeiro (UFRRJ); MARCO ROBERTO BOURG DE MELLO, Universidade Federal Rural do Rio de Janeiro (UFRRJ); GABRIEL BRUN VERGANI, Universidade Estadual Paulista (UNESP); LUCAS MACHADO FIGUEIRA; SERGIO NOVITA ESTEVES, CPPSE; VERONICA SCHINAIDER DO A PEREIRA, CPPSE; PAWEL MIECZYSLAW BARTLEWSKI; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista (UNESP); JOANNA MARIA GONÇALVES SOUZA-FABJAN; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Nonsurgical embryo recovery from estrus-synchronized or superovulated Morada Nova ewes: a feasible strategy for sheep embryo banking. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Biopreservation and Biobanking, v. 19, n. 5, oct. 2021. |
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DOI: |
10.1089/bio.2020.0125 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study assessed the feasibility of in vivo embryo production and nonsurgical embryo recovery (NSER) in Morada Nova ewes (an endangered native Brazilian breed of sheep) subjected to different estrus synchronization and/or superovulation protocols. Ewes received intravaginal sponges soaked with 60 mg medroxyprogesterone acetate (MAP), which were kept in place for six (G6; n = 12), nine (G9; n = 12), or 12 (G12; n = 12) days. Half of the ewes in each group remained estrus synchronized only ((SYNCH)) and the other half was superovulated ((SOV)) with 133 mg porcine follicle-stimulating hormone (pFSH). There were no differences (p > 0.05) in antral follicle counts determined with ultrasonography 60 hours before MAP sponge removal (or at the time of the first pFSH dose) among G6 (6.4 +/- 0.9), G9 (6.2 +/- 0.7), and G12 (5.5 +/- 0.6). Estrus responses and NSER success rates did not vary (p > 0.05) among the three progestin-treatment groups of ewes for either estrus-induced or superovulated animals. The onset of estrus occurred 10-12 hours later (p < 0.01) in G9(SYNCH) ewes compared with G6(SYNCH) and G12(SYNCH,) and the duration of estrus was similar to 19 hours greater (p < 0.01) in G9(SOV) than in G6(SOV). The average duration of the NSER procedure was 32.6 +/- 1.3 minutes. At least one structure was recovered in 85.7% of synchronized and in 87.5% of superovulated ewes. Viable embryo recovery rates were also similar (p > 0.05) for G6 (1.0 +/- 0.3 and 2.5 +/- 1.5), G9 (1.3 +/- 0.5 and 4.8 +/- 2.0), and G12 groups (1.0 +/- 0.3 and 4.8 +/- 2.3; estrus-synchronized and superovulated ewes, respectively). In conclusion, progestogen pretreatment of different durations and NSER can be employed in Morada Nova ewes, resulting in reasonable viable embryo recovery rates in both estrus-synchronized and superovulated animals. Therefore, both techniques are suitable for use in commercial settings as well as small ruminant conservation programs MenosAbstract: This study assessed the feasibility of in vivo embryo production and nonsurgical embryo recovery (NSER) in Morada Nova ewes (an endangered native Brazilian breed of sheep) subjected to different estrus synchronization and/or superovulation protocols. Ewes received intravaginal sponges soaked with 60 mg medroxyprogesterone acetate (MAP), which were kept in place for six (G6; n = 12), nine (G9; n = 12), or 12 (G12; n = 12) days. Half of the ewes in each group remained estrus synchronized only ((SYNCH)) and the other half was superovulated ((SOV)) with 133 mg porcine follicle-stimulating hormone (pFSH). There were no differences (p > 0.05) in antral follicle counts determined with ultrasonography 60 hours before MAP sponge removal (or at the time of the first pFSH dose) among G6 (6.4 +/- 0.9), G9 (6.2 +/- 0.7), and G12 (5.5 +/- 0.6). Estrus responses and NSER success rates did not vary (p > 0.05) among the three progestin-treatment groups of ewes for either estrus-induced or superovulated animals. The onset of estrus occurred 10-12 hours later (p < 0.01) in G9(SYNCH) ewes compared with G6(SYNCH) and G12(SYNCH,) and the duration of estrus was similar to 19 hours greater (p < 0.01) in G9(SOV) than in G6(SOV). The average duration of the NSER procedure was 32.6 +/- 1.3 minutes. At least one structure was recovered in 85.7% of synchronized and in 87.5% of superovulated ewes. Viable embryo recovery rates were also similar (p > 0.05) for G6 (1.0 +/- 0.3 and 2.5 +/- 1.5), G9... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production; Naturalized breed; NSER; Programa de conservação; Raça Morada Nova; Reproductive biotechnologies. |
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Thesagro: |
Ovino; Recurso Genético; Reprodução Animal; Sincronização do Cio; Superovulação. |
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Thesaurus Nal: |
Animal reproduction; Conservation programs; Estrus synchronization; Ewes; Sheep; Small ruminants; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03366naa a2200457 a 4500
001 2137537
005 2023-03-10
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1089/bio.2020.0125$2DOI
100 1 $aARRAIS, A. M.
245 $aNonsurgical embryo recovery from estrus-synchronized or superovulated Morada Nova ewes$ba feasible strategy for sheep embryo banking.$h[electronic resource]
260 $c2021
520 $aAbstract: This study assessed the feasibility of in vivo embryo production and nonsurgical embryo recovery (NSER) in Morada Nova ewes (an endangered native Brazilian breed of sheep) subjected to different estrus synchronization and/or superovulation protocols. Ewes received intravaginal sponges soaked with 60 mg medroxyprogesterone acetate (MAP), which were kept in place for six (G6; n = 12), nine (G9; n = 12), or 12 (G12; n = 12) days. Half of the ewes in each group remained estrus synchronized only ((SYNCH)) and the other half was superovulated ((SOV)) with 133 mg porcine follicle-stimulating hormone (pFSH). There were no differences (p > 0.05) in antral follicle counts determined with ultrasonography 60 hours before MAP sponge removal (or at the time of the first pFSH dose) among G6 (6.4 +/- 0.9), G9 (6.2 +/- 0.7), and G12 (5.5 +/- 0.6). Estrus responses and NSER success rates did not vary (p > 0.05) among the three progestin-treatment groups of ewes for either estrus-induced or superovulated animals. The onset of estrus occurred 10-12 hours later (p < 0.01) in G9(SYNCH) ewes compared with G6(SYNCH) and G12(SYNCH,) and the duration of estrus was similar to 19 hours greater (p < 0.01) in G9(SOV) than in G6(SOV). The average duration of the NSER procedure was 32.6 +/- 1.3 minutes. At least one structure was recovered in 85.7% of synchronized and in 87.5% of superovulated ewes. Viable embryo recovery rates were also similar (p > 0.05) for G6 (1.0 +/- 0.3 and 2.5 +/- 1.5), G9 (1.3 +/- 0.5 and 4.8 +/- 2.0), and G12 groups (1.0 +/- 0.3 and 4.8 +/- 2.3; estrus-synchronized and superovulated ewes, respectively). In conclusion, progestogen pretreatment of different durations and NSER can be employed in Morada Nova ewes, resulting in reasonable viable embryo recovery rates in both estrus-synchronized and superovulated animals. Therefore, both techniques are suitable for use in commercial settings as well as small ruminant conservation programs
650 $aAnimal reproduction
650 $aConservation programs
650 $aEstrus synchronization
650 $aEwes
650 $aSheep
650 $aSmall ruminants
650 $aSuperovulation
650 $aOvino
650 $aRecurso Genético
650 $aReprodução Animal
650 $aSincronização do Cio
650 $aSuperovulação
653 $aIn vivo embryo production
653 $aNaturalized breed
653 $aNSER
653 $aPrograma de conservação
653 $aRaça Morada Nova
653 $aReproductive biotechnologies
700 1 $aMELLO, M. R. B. de
700 1 $aVERGANI, G. B.
700 1 $aFIGUEIRA, L. M.
700 1 $aESTEVES, S. N.
700 1 $aPEREIRA, V. S. do A.
700 1 $aBARTLEWSKI, P. M.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFONSECA, J. F. da
773 $tBiopreservation and Biobanking$gv. 19, n. 5, oct. 2021.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| 1. |  | SOUSA, T. N.; TARAZONA-SANTOS, E. M.; WILSON, D. J.; MADUREIRA, A. P.; FALCAO, P. R. K.; FONTES, C. J. F.; GIL, L. H. S.; FERREIRA, M. U.; CARVALHO, L. H.; BRITO, C. F. A. Genetic variability and natural selection at the ligand domain of the Duffy binding protein in Brazilian Plasmodium vivax populations. Malaria Journal, London, v. 9, n. 334, 2010. 12 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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